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H6254

Hyaluronidase from sheep testes

Name: Hyaluronidase from sheep testes
Brand: SIGMA

Type V, lyophilized powder, ≥1,500 units/mg solid

Synonym(s):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

CAS Number:

37326-33-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

253-464-3

MDL number:

MFCD00131351

EC Number:

3.2.1.35 (BRENDA, IUBMB)

Specific activity:

≥1,500 units/mg solid

Biological source:

sheep testis

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Properties

biological source

sheep testis

Quality Level

200

type

Type V

form

lyophilized powder

specific activity

≥1,500 units/mg solid

mol wt

55 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Description

General description

Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.

Application

Hyaluronidase has been used:

as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines

Biochem/physiol Actions

Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Global Trade Item Number

SKU	GTIN
H6254-1G	04061833800461
H6254-500MG	04061833800485