G2150
L-Glutamine solution
200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma
Synonym(s):
L-Glutamine for cell culture
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75
grade
Hybri-Max™
Quality Level
sterility
sterile-filtered
assay
≥99% (TLC)
form
solution
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
color
clear colorless
shipped in
dry ice
storage temp.
−20°C
SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Gene Information
rat ... Ggt1(116568)
General description
L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Application
The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.
Preparation Note
Prepared in cell culture grade water.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
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wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
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Articles
L-谷氨酰胺在杂交瘤细胞和哺乳动物细胞培养中的重要性和应用
Importance and uses of glutamine in hybridoma and mammalian cell culture
Production of human antibodies from transgenic mice.
C Geoffrey Davis et al.
Methods in molecular biology (Clifton, N.J.), 248, 191-200 (2004-02-19)
Abraham L Moure et al.
Journal of medicinal chemistry, 63(9), 4732-4748 (2020-04-11)
Screening of a GSK-proprietary library against intracellular Mycobacterium tuberculosis identified 1, a thioalkylbenzoxazole hit. Biological profiling and mutant analysis revealed that this compound is a prodrug that is bioactivated by the mycobacterial enzyme MymA. A hit-expansion program including design, synthesis
Glutamine and antioxidants in critically ill patients.
Daren Heyland et al.
The New England journal of medicine, 369(5), 484-485 (2013-08-02)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G2150-20ML | 04061838259530 |