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CHP2NC

Imprint® Ultra Chromatin Immunoprecipitation Kit, Without Controls

ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

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About This Item

NACRES:
NA.54
UNSPSC Code:
41116158
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General description

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.

Application

  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

  • Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
  • Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
  • Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
  • Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Only

Product No.
Description
  • Sodium dodecyl sulfate solution, 10% in 18 megohm water
  • Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • S6576
    ChIP Next Gen Seq Sepharose™
    SDS
  • I8896
    IGEPAL® CA-630, Molecular Biology
    SDS
  • S5150
    Sodium chloride solution, 5 M in H2O, BioReagent, Molecular Biology, suitable for cell culture
    SDS
  • W4502
    Water, Nuclease-Free Water, for Molecular Biology
    SDS
  • I5006
    IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
    SDS
  • I5381
    IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
    SDS
  • A7638
    Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
    SDS
See All (12)

signalword

Danger

target_organs

Central nervous system

Storage Class

5.1A - Strongly oxidizing hazardous materials

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

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Protocols

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

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Imprint Ultra Chromatin IP Kit: for Successful ChIP-Seq with a Low Abundance Transcription Factor

Imprint® Ultra Chromatin IP Kit: for Successful ChIP-Seq with a Low Abundance Transcription Factor

View All Related Content
Comparison of sample preparation methods for ChIP-chip assays.
O'Geen, H., et al.
Biotechniques, 41, 557-557 (2006)
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)
Philippe Collas
Molecular biotechnology, 45(1), 87-100 (2010-01-16)
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest
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Global Trade Item Number

SKUGTIN
CHP2NC-48RXN04061833296073