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C7752

Cytochrome c from equine heart

≥95% based on Mol. Wt. 12,384 basis

Synonym(s):

Cytochrome c from horse heart

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About This Item

CAS Number:
9007-43-6
UNSPSC Code:
12352202
EC Number:
232-700-9
NACRES:
NA.61
MDL number:
MFCD00130890
Form:
powder
Assay:
≥95% (SDS-PAGE), ≥95% based on Mol. Wt. 12,384 basis
Biological source:
horse heart
Mol wt:
12,384
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biological source

horse heart

Quality Level

300

assay

≥95% (SDS-PAGE), ≥95% based on Mol. Wt. 12,384 basis

form

powder

mol wt

12,384

technique(s)

cell culture | mammalian: suitable

suitability

suitable for molecular biology

UniProt accession no.

P00004

storage temp.

−20°C

Gene Information

horse ... CYCS(100053958)

Application

Cytochrome c is used as an inducer of mitochondria-dependent apoptosis in various research studies. The identification and monitoring of oxidation sites in cytochrome c may have implications for assessing oxidative stress-related conditions and diseases. It could potentially be used as a biomarker to evaluate the extent of oxidative damage and the antioxidant capacity of cells and tissues.

Cytochrome c is suitable for use as a component of phosphate buffer for the detection and evaluation of complex IV activity using blue-native gel electrophoresis (BN-PAGE). It may also be used for the detection of extracellular superoxide anion (ECSA) in isolated kidney phagocytes.
The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.

Biochem/physiol Actions

Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell.
Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen

Preparation Note

Prepared with acetic acid without using TCA.

Other Notes

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
098K7000
077K7001
065K7001
102K7053
080K7042

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Protocols

超氧化物歧化酶的酶学测定

在测定超氧化物歧化酶活性时，使用了一种在550 nm处的连续分光光度法进行测定。一个酶单位可将酶偶联系统的细胞色素c还原率抑制50％。

Enzymatic Assay of Superoxide Dismutase

Enzymatic Assay of Superoxide Dismutase

Articles

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins on TSKgel® BioAssist S

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

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Global Trade Item Number

SKUGTIN
C7752-500MG04061835556250
C7752-50MG04061835518036
C7752-5G04061835512324
C7752-100MG04061835556236
C7752-1G04061835556243