ANTI-FLAG^® M2 Affinity Gel

采用M2单克隆抗体4％琼脂糖亲和凝胶进行的FLAG融合蛋白免疫沉淀（IP）实验方案

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.

FLAG®表达系统是一种表达、纯化和检测重组融合蛋白的成熟方法。作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。M2抗体可结合哺乳动物、细菌和植物提取物融合蛋白N端、Met-N端或C端的FLAG®肽。

用于纯化重组蛋白的蛋白纯化技术、试剂和方法包括离子交换、排阻层析和蛋白亲和层析。

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.