G7663
Gelatin blocking buffer
for Western blotting, powder blend
Synonym(s):
blocking buffer
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105300
General description
Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).
Application
Suitable for use as a non-specific blocking agent for Western blots.
Preparation Note
Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
为了特异性地检测固定在固体支持物上的抗原或靶分子，必须将支持物上未被占据的结合位点进行封闭以防止探针和检测分子结合。
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| G7663-1L | 04061833643068 |