LC/MS/MS Analysis of Vitamin D Metabolites on Ascentisยฎ Express F5 (Monitored Ions)
Materials
used together
๊ด๋ จ ์ ํ
25-Hydroxyvitamin D2 solution
50 ฮผg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliantยฎ25-Hydroxyvitamin D3 solution
100 ฮผg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliantยฎ1ฮฑ,25-Dihydroxyvitamin D3 solution
5 ฮผg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliantยฎ1ฮฑ,25-Dihydroxyvitamin D2 solution
5 ฮผg/mL in ethanol, ampule of 1 mL, certified reference material, CerilliantยฎCONDITIONS
column
Ascentis Express F5, 15 cm x 2.1 mm I.D., 2.7 μm particles (53571-U)
mobile phase
[A] 5 mM ammonium formate water; [B] methanol; (25:75, A:B)
flow rate
0.4 mL/min
pressure
5178 psi (357 bar)
column temp.
40 ยฐC
detector
ESI(+), 100-1000 m/z
injection
2 μL, each compound 300 ng/mL in 40:60 (water:methanol)
์ค๋ช
๋ถ์ ๋ฉ๋ชจ
The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity.This application shows that a combination of Ascentis Express F5 with MS detection provided rapid resolution of seven such compounds. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany