HPLC Analysis of Serine Enantiomers on Astecยฎ CHIROBIOTICยฎ TAG
Materials
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L-Serine
from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%CONDITIONS
column
CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 μm particles (14024AST)
mobile phase
[A] water; [B] acetonitrile; (70:30, A:B)
flow rate
1 mL/min
column temp.
25 ยฐC
detector
UV, 210 nm
injection
5 μL
sample
10 mg/mL in methanol
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Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC