HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentisยฎ Express RP-Amide
Materials
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Ascentisยฎ Express RP-Amide, 2.7 ฮผm HPLC Column
2.7 μm particle size, L ร I.D. 15 cm ร 3 mmCONDITIONS
column
Ascentis Express RP-Amide, 15 cm x 3 mm I.D., 2.7 μm particles (53919-U)
mobile phase
[A] water; [B] acetonitrile
gradient
5 to 53% B in 70 min
flow rate
0.4 mL/min
column temp.
35 ยฐC
detector
UV, 240 nm
injection
5 μL
sample
20 mg/L (except terbuthylazin desethyl and terbuthylazine 60 mg/L) in 60:40, water: acetonitrile
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This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of 16 triazine and phenyl urea compounds. Lower column temperature can improve resolution of the critical pair.
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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany