Characterization of ceramic hydroxyapatite surface by inverse liquid chromatography in aquatic systems.

Characterization of ceramic hydroxyapatite surface by inverse liquid chromatography in aquatic systems.

Talanta (2015-11-26)

Karol Kadlec, Katarzyna Adamska, Adam Voelkel

초록

The novel approach for hydroxyapatite (HA) surface characterization was proposed. The main aim of this investigation was to estimate surface properties of HA as a biomaterial in real system i.e. in simulated body fluid (SBF). One of the method, which might be used to reflect the influence of liquid environment on sorption properties of material being surrounded by this liquid, is called inverse liquid chromatography (ILC). The lowercase letters of LFER equation (e, s, a, b, v) served for this characterization. The sorption abilities of examined material were also estimated for two different aqueous mobile phases: deionized water and water solution of 0.1M Na2HPO4. It enabled to observe the change in physiochemical properties of surface, considered in Abraham model, dependence on ions concentration in the mobile phase. Moreover pH of every aquatic solution, normally about 7, was adjusted to 5.5 and 9 to observe the influence of hydrogen and hydroxyl ions concentration on HA sorption properties.

MATERIALS

제품 번호

브랜드

제품 설명

T6791

Sigma-Aldrich

Trizma^® base, BioXtra, pH 10.5-12.0 (1 M in H₂O), ≥99.9% (titration)

S5761

Sigma-Aldrich

Sodium bicarbonate, powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture

T1503

Sigma-Aldrich

Trizma^® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

C5267

Sigma-Aldrich

Calcium phosphate tribasic, 34.0-40.0% Ca basis

60128

Sigma-Aldrich

Potassium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)

71376

Sigma-Aldrich

Sodium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)

21223

Sigma-Aldrich

Hydroxyapatite, purum p.a., ≥90% (as Ca₃(PO₄)₂, KT)

45726

Sigma-Aldrich

Acetic acid, suitable for luminescence, BioUltra, ≥99.5% (GC)

60142

Sigma-Aldrich

Potassium chloride solution, BioUltra, Molecular Biology, ~1 M in H₂O

93362

Sigma-Aldrich

Trizma^® base, BioUltra, Molecular Biology, ≥99.8% (T)

71959

Sigma-Aldrich

Sodium sulfate, BioUltra, anhydrous, ≥99.0% (T)

93350

Sigma-Aldrich

Trizma^® base, 99.7% (T), puriss. p.a.

04238

Sigma-Aldrich

Hydroxyapatite, puriss., meets analytical specification of Ph. Eur., BP, FCC, E341, ≥90% (calculated on glowed substance)

S6297

Sigma-Aldrich

Sodium bicarbonate, BioXtra, 99.5-100.5%

C3161

Sigma-Aldrich

Calcium phosphate tribasic, BioReagent, suitable for plant cell culture, powder

T6066

Sigma-Aldrich

Trizma^® base, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

T6687

Sigma-Aldrich

Tromethamine, meets USP testing specifications

451029

Sigma-Aldrich

Potassium chloride, AnhydroBeads^™, −10 mesh, 99.99% trace metals basis

W292818

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1-Propanol, ≥99%, FG

W292826

Sigma-Aldrich

1-Propanol, natural, ≥98%, FG

279544

Sigma-Aldrich

1-Propanol, anhydrous, 99.7%

449989

Sigma-Aldrich

Potassium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

450006

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

252859

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Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%

93352

Sigma-Aldrich

Trizma^® base, ≥99.0% (T)

109819

Sigma-Aldrich

Propylamine, 98%

P9541

Sigma-Aldrich

Potassium chloride, Molecular Biology, ≥99.0%

P5405

Sigma-Aldrich

Potassium chloride, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%

P9327

Sigma-Aldrich

Potassium chloride solution, 0.075 M, sterile-filtered, BioXtra, suitable for cell culture

T4661

Sigma-Aldrich

Trizma^® base, ≥99.9% (titration), crystalline