G8255
Glutamic-Pyruvic Transaminase from porcine heart
lyophilized powder, ≥75 units/mg protein
동의어(들):
L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
생물학적 소스
Porcine heart
Quality Level
양식
lyophilized powder
특이 활성도
≥75 units/mg protein
구성
Protein, 75-95%
기술
cell culture | mammalian: suitable
색상
light yellow to yellow
외래 활성
L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.1%
저장 온도
−20°C
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일반 설명
Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.
Research area: Cell Signaling
Research area: Cell Signaling
애플리케이션
Glutamic-Pyruvic Transaminase from porcine heart has been used:
- in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate
- to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells
- in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies
생화학적/생리학적 작용
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.
Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.
물리적 형태
Lyophilized powder containing citrate and acetate
제조 메모
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
분석 메모
Protein determined by biuret
기타 정보
One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
Giordano Pula et al.
Journal of biochemical and biophysical methods, 64(3), 167-181 (2005-08-23)
The surface expression of G protein-coupled receptors is regulated by internalization. For many receptors, a constitutive level of internalization in the absence of agonist has been reported. The constitutive internalization of metabotropic glutamate receptor 1a (mGluR1a) has been described, but
A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin
Chen M, et al.
Royal Society of Chemistry Advances, 5, 103557-103562 (2015)
A Comprehensive Guide to Toxicology in Nonclinical Drug Development
Faqi A S
Biomarkers journal., 2017 null
Wei Guo et al.
Cancers, 12(7) (2020-07-15)
Metabolic reprogramming is an essential hallmark of cancer. Besides the "Warburg effect", cancer cells also actively reprogram amino acid metabolism to satisfy high nutritional demands in a nutrient-poor environment. In the glucose-alanine cycle, exogenous alanine taken up by hepatocytes is
M A Mahtab et al.
Bangladesh Medical Research Council bulletin, 38(3), 104-107 (2013-04-02)
Hepatitis B surface antigen (HBsAg) is regarded as sole marker of hepatitis B virus (HBV) infection in Bangladesh and most other developing countries. However, some HBV-negative subjects may harbor HBV DNA and transfusion of their blood may cause HBV infection
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