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Merck

NGLYFL-RO

Roche

N-Glycosidase F

recombinant form of the gene from Flavobacterium meningosepticum

동의어(들):

N-Glycosidase F, PNGase F, Peptide-N-glycosidase F, Peptide-N4-(acetyl-β-glucosaminyl)-asparagine amidase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

EC 번호:
3.5.1.52 (BRENDA, IUBMB)
UNSPSC 코드:
12352204

주요 문서

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기술 서비스
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도움 문의

생물학적 소스

bacterial (Flavobacterium meningosepticum)

Quality Level

100

재조합

expressed in E. coli

결합

(N-linked)

분석

≥90% (SDS-PAGE)

양식

lyophilized

특이 활성도

>25000 units/mg protein

분자량

35.5 kDa

정제법

electrophoresis

포장

pkg of 100 U (11365185001)
pkg of 250 U (11365193001)

제조업체/상표

Roche

저장 조건

(Keep container tightly closed in a dry and well-ventilated place.)

기술

activity assay: suitable

색상

colorless

최적 pH

7.0-8.0

solubility

water: soluble

적합성

suitable for enzyme test

외래 활성


Endoglycosidase F, none detected
a-Fucosidase, present
b-Galactosidase, present
b-N-Acetylhexosaminidase, present
dA(17h ≤100 units, present

저장 온도

2-8°C

관련 카테고리

일반 설명

N-Glycosidase F (PNGase F) is a potent enzyme which hydrolyzes at glycosylamine linkage. It also helps in generating a carbohydrate-free peptide and oligosaccharide with di-N-acetylchitobiose unit.

N-glycosidase F, also known as PNGase F, is an asparagine amidase enzyme derived from Flavobacterium meningosepticum. It is widely used as a valuable tool in protein research to investigate and analyze N-glycosylation.

애플리케이션

N-Glycosidase F has been used for deglycosylation of N-glycoproteins.

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a solution.

생화학적/생리학적 작용

Hydrolyzes all types of N-glycan chains from glycopeptides and glycoproteins unless they carry α1,3-linked core fucose residues present in insect and plant glycoproteins. Free of contaminating proteolytic activities (x = H or sugar[s]) according to current quality control procedures.

물리적 형태

Clear, colorless solution after reconstitution

제조 메모

Storage conditions (working solution): 2 to 8 °C
The reconstituted solution is stable at 2 to 8 °C for at least four weeks.
Dissolving the content in 0.1 ml redist water (100 unit package) or 0.25 ml double-dist. water (250 unit package) respectively, results in a concentration of 100 mM sodium phosphate buffer, 25 mM EDTA, pH 7.2.
Note: N-Glycosidase F, recombinant is also available as solution with 50% glycerol.

기타 정보

For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycoprotein within 1 minute at 37 °C and pH 7.8.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
71758000
71312700
66085400
60812600
66085300

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리 방문

A L Tarentino et al.
Biochemistry, 24(17), 4665-4671 (1985-08-13)
Endo-beta-N-acetylglucosaminidase F (Endo F) and peptide:N-glycosidase F (PNGase F) were purified from cultures of Flavobacterium meningosepticum by ammonium sulfate precipitation followed by gel filtration on TSK HW-55(S). This system separated the two enzymes and provided PNGase F in a high

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