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Merck

W4502

Sigma-Aldrich

Water

suitable for electrophoresis, PCR

Synonym(s):

DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water

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About This Item

Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
231-791-2
MDL number:
MFCD00011332
UNSPSC Code:
12191602
PubChem Substance ID:
24901995
NACRES:
NA.31
Technique(s):
PCR: suitable
electrophoresis: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg

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Product Name

Water, Nuclease-Free Water, for Molecular Biology

vapor density

<1 (vs air)

Quality Level

200

vapor pressure

3 mmHg

sterility

0.1 μm filtered

form

liquid

technique(s)

PCR: suitable
electrophoresis: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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Application

Water has been used-
  • in cDNA synthesis and PCR amplification
  • for the dilution of rumen DNA sample prior to qPCR assay
  • in PCR (polymerase chain reaction) and agarose gel electrophoresis
  • for the elution of RNA during RNA isolation

Features and Benefits

  • Nuclease-Free Water
  • DNase-Free, RNase-Free, Protease- Free
  • Not DEPC Treated
  • 0.1 um Filtered
  • Prepared from Double Distilled and Deionized Water
  • Suitable for use in Molecular Applications

This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).

Preparation Note

This product is prepared from double distilled and deionized water

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0110
RNBL9926
RNBL9241
RNBL9257
RNBL7092

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Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
Skraber S et al
Applied and Environmental Microbiology, 70(6), 3644-3649 (2004)
Presence of viral genomes in mineral water: a sufficient condition to assume infectious risk?
Gassilloud B et al
Applied and Environmental Microbiology, 69(7), 3965-3969 (2003)
Polymorphic exact tandem repeat A (PETRA): a newly defined lineage of mycobacterium tuberculosis in israel originating predominantly in Sub-Saharan Africa.
Freidlin PJ et al
Journal of Clinical Microbiology, 47(12), 4006-4020 (2009)
Effect of sward dry matter digestibility on methane production, ruminal fermentation, and microbial populations of zero-grazed beef cattle.
Hart KJ et al
Journal of Animal Science, 87(10), 3342-3350 (2009)
Gene expression signals involved in ischemic injury, extracellular matrix composition and fibrosis defined by global mRNA profiling of the human left ventricular myocardium.
Gabrielsen A et al
Journal of Molecular and Cellular Cardiology, 42(4), 870-883 (2007)
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