PP0530
GlycoProfile™ 2-AA Labeling Kit
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About This Item
UNSPSC Code:
12352200
EC Number:
204-287-5
NACRES:
NA.32
General description
The GlycoProfile™ labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosylphosphatidylinositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.
Application
While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.
GlycoProfile™ 2-AA Labeling Kit has been used in the labeling of fluorescently derivatized N-glycans from chinese hamster ovary CHO cells.
Legal Information
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- Anthranilic acid (2-AA) 2 x 6
- Dimethyl sulfoxide (DMSO) 2 x 350
- Acetic acid, glacial 2 x 200
- Reductant (sodium cyanoborohydride) 2 x 6
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
302.0 °F - closed cup
flash_point_c
150 °C - closed cup
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Articles
Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.
Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.
Related Content
Glycobiology and Glycoproteomics Brochure
Modulation of IgG1 immunoeffector function by glycoengineering of the GDP-fucose biosynthesis pathway
Kelly RM, et al.
Biotechnology and Bioengineering, 115(3), 705-718 (2018)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PP0530-1KT | 04061836686925 |