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Merck

P9187

Sigma-Aldrich

OPD

SIGMAFAST™, peroxidase substrate, chromogenic, tablet

Synonym(s):

OPD Peroxidase Substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

Key Documents

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Product Name

SIGMAFAST™ OPD, tablet

form

tablet

Quality Level

200

solubility

water: 0.5 tablet/10 mL, clear, colorless

storage temp.

2-8°C

Related Categories

Application

SIGMAFAST™ OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of peroxidase activity in enzyme immunoassays (EIA). OPD is the EIA substrate of choice as it exhibits high sensitivity. EIA applications utilizing OPD may be read in timed assays or stopped with dilute acid solutions for delayed readings.
SIGMAFAST OPD has been used in saliva- virus-like particles (VLP) binding assay. It has also been used to develop enzyme linked immunosorbent assay (ELISA) plates for the detection of rituximab in rat serum samples.

Features and Benefits

SIGMAFAST™ OPD Tablets require no additional buffers or steps to prepare an active substrate solution.

Preparation Note

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing OPD and urea hydrogen peroxide.
One OPD tablet and one urea hydrogen peroxide/buffer tablet, dissolved in 20 ml of water, provides 20 ml of ready-to-use substrate. When the tablet is dissolved in this manner, the final concentrations will be 0.4 mg/ml OPD, 0.4 mg/ml urea hydrogen peroxide, and 0.05 M phosphate-citrate, pH 5.0. Tablets are individually packaged in foil packets.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

This substrate is hygroscopic and light sensitive

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ3691
SLCG4913
SLCC0308
SLBZ5219
SLBS6448

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Subcutaneous absorption of monoclonal antibodies: role of dose, site of injection, and injection volume on rituximab pharmacokinetics in rats
Kagan L, et al.
Pharmaceutical Research, 29(2), 490-499 (2012)
Yoo Min Park et al.
Nano convergence, 7(1), 29-29 (2020-09-02)
The noninvasive early detection of biomarkers for Alzheimer's disease (AD) is essential for the development of specific treatment strategies. This paper proposes an advanced method for fabricating highly ordered and flexible nanopillar-based electrochemical biosensors by the combination of soft/photolithography and
Eliana Spilioti et al.
Environmental geochemistry and health, 39(4), 821-833 (2016-07-23)
Spa resorts are known for thousands of years for their healing properties and have been empirically used for the treatment of many inflammatory conditions. Mud is one of the most often used natural materials for preventive, healing and cosmetic reasons
Gisela Esquerda-Canals et al.
PloS one, 14(5), e0217793-e0217793 (2019-06-01)
The success of the targeting of amyloid-β (Aβ) oligomers through immunotherapy in Alzheimer's disease (AD) mouse models has not been translated into the clinics. The use of single-chain variable fragments (scFvs) has been proposed to prevent the potential severe effects
Elisa Lobato et al.
Ecology and evolution, 7(11), 3645-3656 (2017-06-16)
Oceanic islands share several environmental characteristics that have been shown to drive convergent evolutionary changes in island organisms. One change that is often assumed but has seldom been examined is the evolution of weaker immune systems in island species. The
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간접 ELISA 및 포획 ELISA 실시에 관한 상세한 지침을 제공하는 ELISA 프로토콜 샌드위치 ELISA 및 간접 ELISA 실험을 실시하기 위한 권장 제품과 기법에 대해 자세히 알아봅니다.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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