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L3540

Lowry Reagent

Synonym(s):

Copper(II) tartrate Mix

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
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Quality Level

100

General description

The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.

Application

Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.
For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.

Preparation Note

Reconstitute to 40 mL with deionized water.

Other Notes

Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Corr. 1B

Storage Class

4.1B - Flammable solid hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
078K6155
095K62001
126K6056
103K6010
036K6019

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Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Related Content

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

단백질 정량

다양한 샘플 내의 단백질 농도를 정확히 측정하기 위한 단백질 정량법, 시약 및 면역분석 기술.

Product Information Sheet - L3540
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A simplification of the protein assay method of Lowry et al. which is more generally applicable
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Environmental and genetic perturbations reveal different networks of metabolic regulation
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Global Trade Item Number

SKUGTIN
L3540-1VL04061822419513
L3540-25VL04061838261922