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D3812

DirectLoad™ 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

Synonym(s):

DirectLoad™ 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Step Ladder

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105335
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Quality Level

200

form

(liquid)

usage

sufficient for 100 loads

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s DirectLoad™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
DirectLoad™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s DirectLoad™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
108K1566
068K1279
018K1665
047K1185
086K1958

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Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

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Global Trade Item Number

SKUGTIN
D3812-1VL04061835515967