D0426

DAB with Metal Enhancer

Name: DAB with Metal Enhancer
Brand: SIGMA

SIGMAFAST^™, peroxidase substrate, tablet

Synonym(s):

3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.83

Key Documents

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Product Name

SIGMAFAST^™ DAB with Metal Enhancer, tablet

form

tablet

Quality Level

200

solubility

water: 1 tablet/5 mL

storage temp.

−20°C

Related Categories

Detection Substrates & Enzymes

Protein Biology

Proteins & Enzymes

General description

SIGMAFAST^™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.

Application

SIGMAFAST^™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry, dorsal root ganglion (DRG) neurons and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.

The DAB reaction is enhanced by the addition of CoCl₂ and produces a more intense stain which is resistant to alcohol.

Preparation Note

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Pictograms

GHS08,GHS05,GHS09

Signal Word

Danger

Hazard Statements

H317,H318,H334,H341,H350,H360F,H411

Precautionary Statements

P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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From the Cover: 2.45-GHz Microwave Radiation Impairs Hippocampal Learning and Spatial Memory: Involvement of Local Stress Mechanism-Induced Suppression of iGluR/ERK/CREB Signaling.

Saba Shahin et al.

Toxicological sciences : an official journal of the Society of Toxicology, 161(2), 349-374 (2017-10-27)

Microwave (MW) radiation induced oxidative stress reduces dendritic arborization, spine density and number of hippocampal pyramidal neurons and hence, impair learning and spatial memory through p53-dependent/independent apoptosis of hippocampal neuronal and nonneuronal cells. However, the mechanisms responsible for MW radiation

Testicular atrophy and reproductive quiescence in photorefractory and scotosensitive quail: Involvement of hypothalamic deep brain photoreceptors and GnRH-GnIH system.

Somanshu Banerjee et al.

Journal of photochemistry and photobiology. B, Biology, 175, 254-268 (2017-09-20)

Birds time their daily and seasonal activities in synchronization with circadian and annual periodicities in the environment, which is mainly provided by changes in photoperiod/day length conditions. Photoperiod appears to act at the level of eye, pineal and encephalic/deep brain

Evaluation of Hematobin as a Vaccine Candidate to Control Haematobia irritans (Diptera: Muscidae) Loads in Cattle.

M Breijo et al.

Journal of economic entomology, 110(3), 1390-1393 (2017-04-08)

The horn fly, Haematobia irritans (L.), is a blood-sucking livestock ectoparasite responsible for substantial livestock losses. In the present work, the potential use of recombinant hematobin (HTB), a horn fly salivary protein, as an antigen for cattle vaccination was investigated.

Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture

Cheng M, et al.

Tissue Engineering: Part A, 16(5), 1479-1489 (2010)

Limited developmental neurotoxicity from neonatal inhalation exposure to diesel exhaust particles in C57BL/6 mice.

Keith Morris-Schaffer et al.

Particle and fibre toxicology, 16(1), 1-1 (2019-01-08)

Recent epidemiological studies indicate early-life exposure to pollution particulate is associated with adverse neurodevelopmental outcomes. The need is arising to evaluate the risks conferred by individual components and sources of air pollution to provide a framework for the regulation of

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Protocols

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Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

DAB with Metal Enhancer