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C4482

CelLytic™ Y Cell Lysis Reagent

For yeast cells

Synonym(s):

Yeast cell lysis reagent

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About This Item

NACRES:
NA.56
UNSPSC Code:
41116134
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Quality Level

200

form

solution

General description

CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.

Application

CelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).

Features and Benefits

  • Fast: Cell lysis in just 20 minutes
  • Efficient: Three times more efficient than glass beads
  • Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
  • Gentle: Non-denaturing - preserves biological activity

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

pictograms

Exclamation mark
GHS07

Storage Class

10 - Combustible liquids

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Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2

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Certificates of Analysis (COA)

Lot/Batch Number
018K4026
077K4008
121K4027
104K4107
071K4046

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Articles

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Yeast Cell Lysis Reagents

CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.

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용해 및 단백질 추출

세포 용해 및 단백질 추출 방법은 세제 용해부터 기계적 파쇄법에 이르기까지 다양한 기술을 포함하며 연구에 필요한 요구 사항을 지원합니다.

CelLytic Protein Extraction Reagents
Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

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DNA damage induced nucleotide excision repair in Saccharomyces cerevisiae
Singh RK and Krishna M
Molecular and Cellular Biochemistry, 290(1-2), 103-112 (2006)
Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity
Li R, et al.
Protein Expression and Purification, 96, 1-7 (2014)
The beta6beta7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation
Xu L, et al.
Cellular and Molecular Life Sciences, 75(8), 1445-1459 (2018)

Global Trade Item Number

SKUGTIN
C4482-10X1.5ML04061838248220
C4482-250ML04061836971762
C4482-50ML04061838254993
C4482-1.5ML04061838213266
C4482-500ML04061838213273