Skip to Content
Merck

Key Documents

View All Documentation

03-0187-00

SMC® Human MCP-1 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human MCP-1 in human serum and plasma

Synonym(s):

MCP 1 immunoassay kit, MCP-1 Single Molecule Counting Kit, MCP-1 ultra-sensitive immunoassay, Monocyte Chemoattractant Protein-1 immunoassay kit

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

SMC® Human MCP-1 High Sensitivity Immunoassay Kit,

description

Used for ultrasensitive quantification of Human MCP-1 in human serum and plasma

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

single molecule counting: suitable

input

plasma (K2 EDTA)
serum

UniProt accession no.

P13500

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

General description

Monocyte Chemoattractant Protein-1 (MCP-1), also known as CCL2, is a chemokine that plays a critical role in the recruitment of monocytes to sites of inflammation and injury. It is produced by various cell types, including macrophages, endothelial cells, and fibroblasts, in response to inflammatory stimuli. MCP-1 is involved in several physiological and pathological processes, including atherosclerosis, chronic inflammatory diseases, and tissue repair.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

  • Breakthrough-level sensitivity for translational research: Supports early-stage biomarker identification and immune profiling with unmatched precision.
  • Wide Quantification Range: Captures MCP-1 levels across both baseline and elevated inflammatory states, ideal for diverse sample types.
  • Streamlined ELISA Workflow with SMC® Enhancements: Uses a familiar sandwich immunoassay format with a unique elution step for digital molecule counting.
  • Illuminate Monocyte Migration Pathways: MCP-1 is a key chemokine in immune cell recruitment; this kit helps quantify its role in inflammation and tissue infiltration.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 150 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 80-400,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 102% Plasma: 94%
  • Recovery: Serum: 104% Plasma: 101%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

MCP-1 was not cross-reactive with rat, mouse, or canine samples.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Power of High Sensitivity Immunology Assays| SMC® Technology

Use the power of high sensitivity immunology assays with SMC® technology to precisely characterize low-abundant cytokines like those from Th1/Th2 and Th17 cells.

고감도 면역학 분석의 힘 | SMC® 기술

SMC® 기술을 활용하여 Th1/Th2 및 Th17 세포와 같이 낮은 수준의 사이토카인을 정확하게 특성화함으로써 고감도 면역학 분석의 힘을 통해 면역학 연구를 향상시키는 방법을 알아보세요.

Protocol- 03-0187-00

Global Trade Item Number

SKUGTIN
03-0187-0004061841929932