Affinity Chromatography Resins
Affinity chromatography separates complex mixtures through highly selective biochemical interactions. This widely used purification technology efficiently separates a target molecule from impurities. Our range of affinity resins are designed for high throughput and cost-effective purification, from process development through large-scale manufacturing.
Section Overview
Protein A Affinity Resins
Eshmuno® A is a high-capacity resin for the purification of Fc-containing proteins, such as monoclonal antibodies (mAbs). This resin can be sanitized under acid and alkaline conditions.
Affinity Resins for Plasma Purification
Eshmuno® P anti-A and anti-B resins effectively remove anti-A and anti-B isoagglutinin antibodies in plasma-derived Immunoglobulin (Ig) processes, which has been associated with increased patient risk for hemolysis.
Affinity Resins for Protein Purification
Fractogel® Metal Chelate resin offers efficient and cost-effective separation of proteins and peptides, especially proteins with exposed histidine, cysteine, and tryptophan residues, including histidine-tagged proteins.
Support for qualification, risk assessment, and process optimization
Complementing our product portfolio, the Emprove® Program provides three types of dossiers to support different stages of development and manufacturing operations such as qualification, risk assessment, and process optimization. The dossiers consolidate comprehensive product-specific testing data, quality statements, and regulatory information in a readily available format to simplify your compliance needs.
Related Resources
- Guide: Biochromatography Portfolio
Bulk resin, membranes and prepacked columns.
- Guide: Chromatography Purification Product Selection
Chromatography product selection guide.
- Data Sheet: Eshmuno® A Resin
Eshmuno® A media is a rigid, high-capacity, acid and alkaline resistant Protein A affinity chromatography media for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies.
- Data Sheet: Eshmuno® P anti-A and anti-B Resins
High performance, acid and alkaline resistant affinity chromatography resins designed for the removal of anti-A and anti-B antibodies from plasma-derived immunoglobulin (Ig).
- Data Sheet: Fractogel®Chromatography Resin
Fractogel® resins consist of synthetic methacrylate based polymeric beads providing excellent pressure stability resulting in high flow rates. Depending on your purification application, we can offer resin sized M-type beads with a particle size of 40–90 μm and small S-type beads with a particle size in the range of 20–40 μm.
- Application Note: Increase your Eshmuno® A resin lifetime with Ethylene Glycol Emprove® Evolve
Sodium hydroxide (NaOH) is a standard cleaning agent for protein A chromatography resins. Typically, a concentration of 0.1 mol/L NaOH is used. The inclusion of 20% ethylene glycol in a 0.1 mol/L NaOH cleaning solution increases the lifetime of Eshmuno® A columns significantly.
- Application Note: Detection and Quantification of Leached Protein A from Eshmuno® A Resin
Using Cygnus F610 Protein A Mix-N-Go™ ELISA Kit.
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