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B5655

BCIP®/NBT

SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet

Synonym(s):

BCIP/NBT Substrate, BCIP®/NBT Alkaline Phosphatase Substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83
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Product Name

SIGMAFAST™ BCIP®/NBT, tablet

form

tablet

Quality Level

200

solubility

water: 10 mL, clear, yellow

storage temp.

−20°C

Application

SIGMAFAST BCIP®/NBT has been used as a substrate
  • for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
  • for alkaline phosphatase in the histochemical staining of primary osteoblasts
  • for alkaline phosphatase staining in bone marrow stromal cells

Preparation Note

Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Storage Class

11 - Combustible Solids

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ2290
098K8205
038K8203
087K8222
047K8218

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Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Guide: Expansion & Differentiation of Human Mesenchymal

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Andriy Didovyk et al.
ACS synthetic biology, 6(12), 2198-2208 (2017-08-11)
Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create
Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells
Teuton JR and Brandt CR
Journal of Virology, 81(8), 3731-3739 (2007)
MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
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Global Trade Item Number

SKUGTIN
B5655-5TAB04061835553952
B5655-25TAB04061835553945