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T3073

Tissue Preparation Solution

30 mL sufficient for 1,000 extractions

Synonym(s):

Direct PCR tissue preparation solution, Extract-N-Amp™ Tissue Preparation Solution, REDExtract-N-Amp™ Tissue Preparation Solution, SYBR® Green Extract-N-Amp™ Tissue Preparation Solution

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About This Item

UNSPSC Code:
12352200
EC Number:
254-457-8
NACRES:
NA.55
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Quality Level

200

usage

30 mL sufficient for 1,000 extractions, sufficient for 1000 reactions

feature

Direct PCR

technique(s)

PCR: suitable

input

crude DNA

shipped in

wet ice

storage temp.

−20°C

General description

The Tissue Preparation Solution is intended for use with the Extract-N-Amp™ Tissue PCR kits, REDExtract-N-Amp™ Tissue PCR kits, and SYBR® Green Extract-N-Amp Tissue PCR kits. This product is intended to supplement the kit reagents, where there are many extractions.

Application

Tissue preparation solution has been used:
  • in combining segments of tails for genotyping
  • to extract genomic DNA from zebrafish embryos/fin tissues
  • to extract DNA from ear tags, tail tips and embryonic yolk sacs of mouse embryo
  • for extraction of DNA from fungal spores and mycelia

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
047K86142
107K86251
028K86191
123K6022
123K6021

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Protocols

REDExtract-N-Amp™ Tissue PCR Kit Protocol

Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

Protokoll für das REDExtract-N-Amp™ Gewebe-PCR-Kit

Das Protokoll des Extract-N-Amp-Kits bietet eine schnelle DNA-Extraktionsmethode, die in 15 Minuten zu einer PCR-bereiten Probe führt. Der optimierte PCR ReadyMix sorgt für eine erfolgreiche und saubere Amplifikation. Der RED-Ladefarbstoff ermöglicht die Probenrückverfolgung.

N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
Soh BS
Cell Research, 24(12), 1420-1432 (2014)
Titilayo D O Falade et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 33(7), 1241-1253 (2016-06-07)
In vitro experimental environments are used to study interactions between microorganisms, and to predict dynamics in natural ecosystems. This study highlights that experimental in vitro environments should be selected to match closely the natural environment of interest during in vitro
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
View All Related Papers

Global Trade Item Number

SKUGTIN
T3073-.3ML04061838250131
T3073-30ML04061833768112
T3073-3ML04061838101266