Key Documents

View All Documentation

S8140

SSPE Buffer

Name: SSPE Buffer
Brand: SIGMA

for Northern and Southern blotting, powder blend

Synonym(s):

Saline-Sodium Phosphate-EDTA Buffer

Select a Size

Change View

About This Item

NACRES:

NA.25

UNSPSC Code:

41105319

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

grade

Molecular Biology

Quality Level

200

form

powder blend

pH

7.2-7.6 (20 × in water, high purity, ambient)

solubility

water, high purity: 20 ×, clear, colorless

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

Description

General description

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

Application

Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).

Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).

Features and Benefits

Easy to reconstitute powder
Multi-purpose use in hybridizations

Preparation Note

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Still not finding the right product?

Explore all of our products under SSPE Buffer

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern und Southern Blot Protokolle und Einführung

Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.

Microfluidic-based enzymatic on-chip labeling of miRNAs

Sonja Vorwek

New Biotechnology (2008)

3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

Igor V. Kutyavin

Nucleic Acids Research, 28(2) (2000)

Global Trade Item Number

SKU	GTIN
S8140-1L	04061838061102
S8140-100ML	04061835509591