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S8015

SSC Buffer

for Northern and Southern blotting, powder blend

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105319
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grade

Molecular Biology

form

powder blend

pH

6.9-7.1 (20-25 °C, 20 ×)

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

General description

SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.

Application

Suitable for use in washing buffer for Northern and Southern hybridization procedures.

Features and Benefits

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Preparation Note

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
117K8214
096K8208
083H6718
056H8855
111H6756

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern und Southern Blot Protokolle und Einführung

Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.

A J Bogers et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 2(3), 160-166 (1988-01-01)
To identify risk factors for hospital mortality after postinfarction left ventricular aneurysmectomy (PILVA), 83 consecutive cases of PILVA were studied in a retrospective analysis. Hospital mortality was 14.4%. Most of the factors correlated with hospital mortality were associated with poor
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of

Global Trade Item Number

SKUGTIN
S8015-1L04061836960490