DUO92101
Duolink® In Situ Red Starter Kit Mouse/Rabbit
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About This Item
Quality Level
product line
Duolink®
technique(s)
proximity ligation assay: suitable
fluorescence
λex 594 nm; λem 624 nm (Texas Red®; Zeiss Filter set 31)
suitability
suitable for fluorescence
storage temp.
−20°C
Application
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
The Duolink® In Situ Red Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
Kit Components Also Available Separately
- Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)
signalword
Danger
wgk
WGK 3
Hazard Classifications
Aquatic Chronic 2 - ED ENV 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
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Protocols
Questo protocollo si usa per la rivelazione mediante immunofluorescenza di proteine presenti nelle cellule e nei tessuti.
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Articles
Scopri come funziona la tecnologia del saggio di ligazione di prossimità e come il kit per verificare l’interazione tra proteine permetta di confermare in situ la presenza di dimerizzazione di EGFR-HER2 indotta da EGF.
Décryptez la technologie des essais de ligature de proximité et découvrez comment notre kit de contrôle des interactions protéiques permet de confirmer la détection in situ d'une dimérisation EGFR/HER2 induite par l'EGF.
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
Related Content
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Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
Global Trade Item Number
| SKU | GTIN |
|---|---|
| DUO92101-1KT | 04061833592199 |