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D4418

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

Synonym(s):

DAB, DAB tablets, Immunohistology substrate

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About This Item

CAS Number:
91-95-2
UNSPSC Code:
12352204
PubChem Substance ID:
24893872
NACRES:
NA.83
MDL number:
MFCD00007725
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Product Name

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 15 mL

Quality Level

200

form

tablet

solubility

water: 1 tablet/5 mL

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Preparation Note

Each tablet set dissolved in 15 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

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Certificates of Analysis (COA)

Lot/Batch Number
097K8208
056K8208
073K8206
065K8206
054K8209

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Protocols

Lisi delle cellule ed estrazione delle proteine per il saggio di Western blotting

Protocollo per preparare campioni di tessuti o di cellule in coltura per la lisi cellulare e l’estrazione efficiente delle proteine da utilizzare nel saggio di Western blotting.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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Global Trade Item Number

SKUGTIN
D4418-50SET04061835572656
D4418-5SET04061835572663