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A4464

Enhanced Avian Reverse Transcriptase [eAMV™ RT]

For reverse transcription at higher temperatures & rare mRNAs

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.55
MDL number:
MFCD01634820
Concentration:
20 units/μL
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Quality Level

200

form

crystalline powder

usage

sufficient for 50 reactions

feature

dNTPs included: no, hotstart: no

concentration

20 units/μL

technique(s)

RT-PCR: suitable, cDNA synthesis: suitable

color

colorless

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

General description

eAMV™ Reverse Transcriptase is an enhanced form of Avian Myeloblastosis Virus (AMV) RT that synthesizes a DNA strand complementary to RNA, DNA, or an RNA:DNA hybrid. This exceptionally robust AMV RT has greater thermostability than standard AMV or Moloney murine leukemia virus(M-MLV) reverse transcriptase. eAMV™ RT is an ideal enzyme for producing high-quality full-length cDNA from total RNA or poly(A)+ RNA and is also efficient at transcribing long targets.

Application

Enhanced Avian (eAMV) Reverse Transcriptase is a highly purified, avian myeloblastosis virus reverse transcriptase (AMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT).

This exceptionally robust AMV-RT has an enhanced ability to detect low abundance messages, transcribe through difficult secondary structure at elevated temperatures (up to 65 °C), and transcribe mRNA templates up to 14.1 kb.
Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.
Enhanced Avian Reverse Transcriptase [eAMV™ RT] has been used for reverse transcription of total RNA to synthesize cDNA during quantitative reverse transcription PCR (RT-qPCR) analysis.

Biochem/physiol Actions

Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.

Features and Benefits

  • Greater sensitivity for low abundance mRNA
  • Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
  • Efficient generation of full-length cDNA, up to 14.1 kb
  • Produces first strand cDNA ready for PCR amplification

Packaging

Provided with a vial of 10× reaction buffer.

Other Notes

One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)12-18 as a primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
125K6106
015K6120
059H9027

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Articles

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Related Content

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

RT-qPCR (PCR quantitative après transcription inverse)

La RT-qPCR, ou PCR quantitative après transcription inverse, combine les effets de la transcription inverse et de la PCR quantitative, ou PCR en temps réel, pour amplifier et détecter des cibles spécifiques. La RT-qPCR trouve de multiples applications, notamment dans la quantification du niveau d'expression génique, la validation de l'interférence par ARN ou la détection d'agents pathogènes comme les virus.

RT-qPCR – Quantitative PCR mit reverser Transkription

In der RT-qPCR oder quantitativen PCR mit reverser Transkription werden die Effekte der reversen Transkription und der quantitativen PCR oder Echtzeit-PCR für die Amplifikation und den Nachweis spezifischer Ziele kombiniert. Die RT-qPCR wird in vielen Anwendungen eingesetzt, darunter die Quantifizierung der Genexpressionsniveaus, die Validierung der RNA-Interferenz (RNAi) und der Nachweis von Krankheitserregern wie Viren.

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Molecular Cloning: A Laboratory Manual, 5-5 (1989)
C Tosh et al.
Acta virologica, 41(3), 153-155 (1997-06-01)
A method of reverse transcription (RT) and polymerase chain reaction (PCR) amplification of 1D (VP1) gene of foot-and-mouth disease (FMD) virus using one reaction mixture containing both avian myeloblastosis virus (AMV) reverse transcriptase (RTase) and Tfl DNA polymerase is described.
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Calcium signaling can elicit different pathways involved in an extreme variety of biological processes. Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology. The Ca2+-ATPase
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Global Trade Item Number

SKUGTIN
A4464-1KU04061833374504