Skip to Content
Merck

Key Documents

View All Documentation

L3540

Lowry Reagent

Synonym(s):

Copper(II) tartrate Mix

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

100

General description

The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.

Application

Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.
For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.

Preparation Note

Reconstitute to 40 mL with deionized water.

Other Notes

Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Corr. 1B

Storage Class

4.1B - Flammable solid hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
078K6155
095K62001
126K6056
104K6237
095K6200

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Related Content

Product Information Sheet - L3540
Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Quantification des protéines

Méthodes, réactifs et technologies de dosage immunologique permettant de mesurer avec précision la concentration en protéines dans divers échantillons.

View All Related Content
A simplification of the protein assay method of Lowry et al. which is more generally applicable
Peterson GL
Analytical Biochemistry, 83(2), 346-356 (1977)
Environmental and genetic perturbations reveal different networks of metabolic regulation
Greenberg A, et al.
Molecular Systems Biology, 7(1), 563-563 (2011)
Raffinose, a plant galactoside, inhibits Pseudomonas aeruginosa biofilm formation via binding to LecA and decreasing cellular cyclic diguanylate levels
Kim HS, et al.
Scientific reports, 6(1), 25318-25318 (2016)
View All Related Papers

Global Trade Item Number

SKUGTIN
L3540-1VL04061822419513
L3540-25VL04061838261922