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MilliporeSigma

Y1003

2x YT medium

Liquid microbial growth medium

Synonyme(s) :

2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium

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A propos de cet article

NACRES:
NA.85
UNSPSC Code:
41106225
Service technique
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grade

Molecular Biology

Quality Level

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.5

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

2xYT liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Other Notes

16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl


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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Victor V Kuprianov et al.
Virus research, 284, 197984-197984 (2020-04-24)
Hepatitis C virus (HCV) can cause chronic infection and evade the immune response. The generation and maintenance of an effective T-cell response is important for immune-mediated control of HCV infection. The purpose of this study was to obtain recombinant mosaic
Hong-Yi Kuo et al.
Glycobiology, 26(2), 155-165 (2015-10-09)
Galectin-3 is a ubiquitous lectin exerting multiple cellular functions such as RNA splicing, protein trafficking and apoptosis. Its expression is positively correlated with the poor prognosis in lung cancer patients. Galectin-3 can promote cancer progression through its effects on cell
Natalay Kouprina et al.
Current protocols, 1(8), e207-e207 (2021-08-10)
Here we describe an updated TAR cloning protocol for the selective and efficient isolation of any genomic fragment or gene of interest up to 280 kb in size from genomic DNA. The method exploits the special recombination machinery of the



Numéro d'article de commerce international

RéférenceGTIN
Y1003-500ML04061837587818