PF0100
Protease Fluorescent Detection Kit
high sensitivity assay
Synonyme(s) :
Protease Assay Kit
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NACRES:
NA.26
UNSPSC Code:
12352202
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Detection limit: approx. 50 ng trypsin
Protease Fluorescent Detection Kit has been used:
- in the detection of proteolytic activity using casein based protease assay
- for testing the extracellular proteolytic activity of fungal strains
- for quantifying mitochondrial protease activity
- for measuring the protease production from soil solution
Preparation Note
Supplied with sufficient reagents for up to 200 one mL assays.
The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.
The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.
Analysis Note
Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).
Composants de kit seuls
Réf. du produit
Description
- Incubation buffer 25 mL
- FITC-casein solution 5 mL
- Assay buffer 200 mL
- Protease standard (trypsin) 20 μg
- FITC control 50 mg
- Trichloroacetic acid (TCA) solution 30 mL
signalword
Danger
hcodes
target_organs
Respiratory system
Classe de stockage
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1 - Skin Sens. 1 - STOT SE 3
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Protocoles
Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.
Articles
Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.
Contenu apparenté
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Martin H Kunzmann et al.
Molecular bioSystems, 8(11), 3061-3067 (2012-09-20)
α-Alkylidene-γ-butyrolactones are quite common in nature and exhibit a broad spectrum of biological activities. We therefore synthesized a small library of xanthatine inspired α-alkylidene-γ-butyrolactones to screen non-pathogenic and uropathogenic E. coli strains by activity based protein profiling (ABPP). The identified
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| PF0100-1KT | 04061834413271 |