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M8787

Chlorure de magnésium solution

PCR Reagent, 25 mM MgCI2 solution for PCR

Synonyme(s) :

PCR optimization

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A propos de cet article

Formule linéaire :
MgCl2
Numéro CAS:
7786-30-3
Poids moléculaire :
95.21
NACRES:
NA.52
PubChem Substance ID:
57654422
UNSPSC Code:
12352302
MDL number:
MFCD00011106
Form:
liquid
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Quality Level

200, 300

form

liquid

packaging

vial of 1.5 mL

concentration

25 mM±1 mM

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture
diagnostic assay manufacturing

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

General description

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Application

Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression
Suitable for optimization of polymerase chain reactions.

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Classe de stockage

12 - Non Combustible Liquids

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Not applicable

flash_point_c

Not applicable

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Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
038K60881
048K6031
038K6088
127K6138
057K60551

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Protocoles

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Protocole de qPCR universel avec SYBR Green

Notre protocole de qPCR avec SYBR Green est conçu pour quantifier précisément l'expression génique ou des réactions de RT-PCR.

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Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.

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KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

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Data Sheet - M8787
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Numéro d'article de commerce international

RéférenceGTIN
M8787-1VL04061834063629
M8787-5ML04061838262387
M8787-5VL04061833297025