I6529
IMDM
With sodium bicarbonate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonyme(s) :
IMDM
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Milieu de Dulbecco modifié par Iscove, With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.
Application
Iscove′s Modified Dulbecco′s Medium has been used to culture:
- human hepatocellular carcinoma (HCC) cell line HepG2 as monolayers
- to culture synovial tissue samples
- bacterial strain inoculum for stimulation of immune cell experiments
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Classe de stockage
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Ram C Shankaraiah et al.
Oncogene, 38(45), 7035-7045 (2019-08-15)
Metformin is a hypoglycaemic agent used to treat type 2 diabetes mellitus (DM2) patients, with a broad safety profile. Since previous epidemiological studies had shown that the incidence of hepatocellular carcinoma (HCC) decreased significantly in metformin treated DM2 patients, we
Patricia López et al.
PloS one, 6(9), e24776-e24776 (2011-10-04)
In this work we analyzed the immune activation properties of different Bifidobacterium strains in order to establish their ability as inductors of specific effector (Th) or regulatory (Treg) responses. First, we determined the cytokine pattern induced by 21 Bifidobacterium strains
Christine Bourgeois et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(25), 8691-8696 (2008-06-20)
A model of chemical thymectomy by inducible Rag ablation was used to study peripheral T cell homeostasis. Induction of Rag ablation was efficient and complete, leading to cessation of thymic T cell production within 3-4 weeks. The decay of peripheral
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| I6529-500ML | 04061837847240 |