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H8651

HEPES sodium salt

Name: HEPES sodium salt
Brand: SIGMA

BioXtra, ≥99.0% (titration)

Synonyme(s) :

Acide 4-(2-hydroxyéthyl)pipérazine-1-éthanesulfonique sodium salt, Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique) sodium salt

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A propos de cet article

Formule empirique (notation de Hill) :

C₈H₁₇N₂NaO₄S

Numéro CAS:

75277-39-3

Poids moléculaire :

260.29

NACRES:

NA.25

PubChem Substance ID:

24895527

UNSPSC Code:

12161700

EC Number:

278-169-7

MDL number:

MFCD00036463

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Propriétés

product line

BioXtra

assay

≥99.0% (titration)

form

powder

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

pH

10.0-12.0 (1 M in H₂O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H₂O: 1 M, clear, colorless

anion traces

chloride (Cl^-): ≤0.05%, sulfate (SO₄^2-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH₄⁺: ≤0.05%, Pb: ≤0.001%, Zn: ≤0.0005%

absorption

≤0.2 at 260 in H₂O at 1 M, ≤0.2 at 280 in H₂O at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Other Notes

Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table.

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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Contenu apparenté

HEPES Sodium Salt Specification Comparison

Nitric oxide reversibly inhibits the epidermal growth factor receptor tyrosine kinase.

C Estrada et al.

The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)

Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor

Direct determination of free triiodothyronine (T3) in undiluted serum by equilibrium dialysis/radioimmunoassay (RIA).

I J Chopra et al.

Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)

We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC

Hydrogen ion buffers for biological research.

Good NE, et al.

BioChemistry: An Indian Journal, 5, 467-477 (1996)

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Numéro d'article de commerce international

Référence	GTIN
H8651-100G	04061833810934
H8651-25G	04061833810989
H8651-500G	04061833811092