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MilliporeSigma

H6648

Solution saline équilibrée de Hank solution

Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

HBSS

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A propos de cet article

NACRES:
NA.75
UNSPSC Code:
12352207
Service technique
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Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

shipped in

ambient

storage temp.

room temp

General description

The essential function of Hanks′ Balanced Salt solution is to maintain pH as well as osmotic balance. It also provides water and essential inorganic ions to cells.
Formulation: click here.

Application

Hanks′ Balanced Salt solution has been used:

  • to study the degradation and cytotoxic effects of the composite material and the ion and/or magnetite release from the scaffolds.
  • to measure the stability of the magnetic scaffold material in bone tissue engineering.
  • in Fluorometric Measurements of intracellular calcium levels [Ca2+]
  • for isolating polymorphonuclear leukocytes (PMN) from umbilical cord blood
  • For assessing airway inflammation in mice

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.


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Classe de stockage

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Protocoles

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Technical information for working with bovine aortic endothelial cells including thawing, subculturing and cryopreservation.

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

Voir tous les protocoles

N Bock et al.
Acta biomaterialia, 6(3), 786-796 (2009-10-01)
In recent years, interest in tissue engineering and its solutions has increased considerably. In particular, scaffolds have become fundamental tools in bone graft substitution and are used in combination with a variety of bio-agents. However, a long-standing problem in the
Andreas Koutroulis et al.
Scientific reports, 9(1), 19019-19019 (2019-12-15)
Tricalcium silicate (TCS)-based materials produce calcium hydroxide as a byproduct of their hydration reaction. The present study investigated whether calcium ion release (CIR) affects their biological and antimicrobial properties when used as pulp protection materials. The effect of incorporation of
A Tampieri et al.
Nanotechnology, 22(1), 015104-015104 (2010-12-08)
Magnetic bio-hybrid porous scaffolds have been synthesized, nucleating nano-apatite in situ on self-assembling collagen, in the presence of magnetite nano-particles. The magnetic phase acted as a sort of cross-linking agent for the collagen, inducing a chemico-physical-mechanical stabilization of the material



Numéro d'article de commerce international

RéférenceGTIN
H6648-500ML04061833801604
H6648-100ML04061833801574
H6648-1L04061833801581
H6648-6X1L04061833801628
H6648-6X500ML04061833801697