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D5042

123 bp DNA Ladder

for DNA electrophoresis

Synonyme(s) :

DNA Ladder for DNA electrophoresis, DNA marker

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A propos de cet article

NACRES:
NA.25
UNSPSC Code:
41105335
Service technique
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Quality Level

100, 200

form

liquid

usage

100 uses (0.1 mg size), 250 uses (250 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Application

123 bp DNA Ladder has been used as a size marker in random amplified polymorphic DNA-polymerase chain reaction (PCR) analysis. It has also been used as a molecular marker in gel electrophoresis.

Other Notes

Contains 35 fragments, 123 - 4182 bp
For optimal resolution, the recommended agarose gel concentration is 1.5%.
Sigma′s 123 bp DNA Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 0.1 mM EDTA.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

flash_point_f

No data available

flash_point_c

No data available

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
128K1396
098K1558
117K1298
047K1459
126K1059

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Articles

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

J L Hartley et al.
Gene, 13(4), 347-353 (1981-05-01)
A method for self-ligation of DNA segments, which is based on the rotational non-equivalence of ends produced by AvaI cleavage, has been developed. Using this method and an initiator molecule to encourage the formation of long polymers, we have obtained
Gene splicing and mutagenesis by PCR-driven overlap extension
Heckman KL and Pease LR
Nature Protocols, 2(4), 924-932 (2007)
E J Gubbins et al.
The Journal of biological chemistry, 255(18), 8655-8662 (1980-09-25)
The organization and sequence of the rat preprolactin gene has been investigated. Analysis of two different plasmids containing pituitary cDNA inserts has provided the complete 681-nucleotide coding sequence of preprolactin as well as 17 nucleotides preceding the initiation codon and
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Numéro d'article de commerce international

RéférenceGTIN
D5042-250UG04061835572717
D5042-.1MG04061838255181