C0773
Collagénase from Clostridium histolyticum
high purity, purified by chromatography, Type VII, ≥4 FALGPA units/mg solid, lyophilized powder, ≥700 CDU/mg solid (CDU = collagen digestion units)
Synonyme(s) :
Clostridiopeptidase A
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
Specific activity:
≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)
Service technique
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Laissez-nous vous aiderQuality Level
type
Type VII
form
lyophilized powder
specific activity
≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)
mol wt
68-130 kDa
purified by
chromatography
composition
Protein, ≥85% biuret
solubility
TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C
foreign activity
caseinase and clostripain ≤1 units/mg protein
storage temp.
−20°C
Application
Collagenase has been used in the preparation of arterial tissue for the study of advanced glycosylation end products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.
Biochem/physiol Actions
La collagénase est activée à l′aide de quatre atome-grammes de calcium par mole d′enzyme. Elle est inhibée par l′acide éthylèneglycol-bis(bêta-aminoéthyléther)-N,N,N′,N′-tétraacétique, le bêta-mercaptoéthanol, le glutathion, l′acide thioglycolique et la 8-hydroxyquinoline.
Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. Optimal pH ranges from 6.3-8.8. The enzyme recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.
Physical form
Lyophilized powder containing calcium chloride
Preparation Note
A stock solution may be prepared by dissolving 0.05 - 0.1 mg of collagenase in one mL of 50 mM TES buffer containing 0.36 mM calcium chloride (TESCA), pH 7.4 at 37 °C. Use cold buffer.
Please note the material will not dissolve completely and few sediments may be seen. The sediments do not affect the activity of the solution.
Other Notes
Une unité de digestion de collagène (UDC) libère une quantité de peptides à partir du collagène d′un tendon d′Achille de bovin équivalant, en termes de couleur avec la méthode à la ninhydrine, à 1,0 μmole de leucine en 5 heures à pH 7,4 et à 37 °C en présence d′ions calcium. Une unité d′hydrolyse de FALGPA hydrolyse 1,0 μmole de furylacryloyl-Leu-Gly-Pro-Ala par minute à 25 °C. Une unité de protéase neutre hydrolyse la caséine et produit une couleur équivalente à 1,0 μmole de tyrosine en 5 heures à pH 7,5 et à 37 °C. Une unité de clostripaïne hydrolyse 1,0 μmole de BAEE par minute à pH 7,6 et à 25 °C en présence de DTT.
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protocoles
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
Contenu apparenté
Sabrina Patzelt et al.
Frontiers in immunology, 12, 812627-812627 (2022-02-25)
Mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by autoantibodies against the basal membrane zone of skin and surface-close epithelia and predominant mucosal lesions. The oral cavity and conjunctivae are most frequently affected, albeit clinical manifestations can also
Chunyan Wang et al.
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 29(5), 104748-104748 (2020-03-13)
Intracerebral hemorrhage (ICH) is a disease that threatens human health due to its high morbidity and mortality. On behalf of finding the better methods in the treatment of ICH, researchers pay more attention to a new technology which is finding
Teemu K Långsjö et al.
Cells, tissues, organs, 172(4), 265-275 (2003-02-05)
The purpose of this study was to compare the ability of electron-microscopic (EM) stereology with quantitative polarized light microscopy (PLM) and biochemical collagen (hydroxyproline) and crosslink (pyridinoline) analyses to detect changes in the superficial collagen network of bovine articular cartilage
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| C0773-15KU | 04061833452073 |
| C0773-60KU | 04061832699844 |
| C0773-1.5KU | 04061833452066 |
| C0773-30KU | 04061832699820 |
| C0773-3KU | 04061832699837 |
| C0773-7.5KU | 04061832699851 |