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21888

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

Name: 5(6)-Carboxyfluorescein diacetate <I>N</I>-succinimidyl ester
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonyme(s) :

CFSE

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A propos de cet article

Formule empirique (notation de Hill) :

C₂₉H₁₉NO₁₁

Numéro CAS:

150347-59-4

Poids moléculaire :

557.46

NACRES:

NA.32

PubChem Substance ID:

57647951

UNSPSC Code:

12352108

MDL number:

MFCD00037466

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Propriétés

Quality Level

200

description

suitable as fluorogenic substrate for esterases

product line

BioReagent

assay

≥90% (HPLC)

form

solid

mol wt

557.46 g/mol

color

almost white to yellow

mp

152-154 °C (lit.)

solubility

DMF: soluble, DMSO: soluble

fluorescence

λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

Description

General description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.

Application

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation.

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

5-Carboxyfluorescein diacetate N-succinimidyl ester has been used:

to label cells for flow cytometry
in lymphocyte proliferation assay
to determine the intracellular or intra-organellar pH in chloroplasts
to label Leishmania amazonensis and Leishmania major

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Numéro d'article de commerce international

Référence	GTIN
21888-25MG-F	04061838775702
21888-100MG-F	04061838775696