Principaux documents

Voir toute la documentation

E4884

Acide éthylènediaminetétraacétique disodium salt dihydrate

Name: Acide éthylènediaminetétraacétique disodium salt dihydrate
Brand: SIAL

ACS reagent, 99.0-101.0%

Synonyme(s) :

EDTA disodium salt, EDTA-Na₂, Edétate disodium salt dihydrate, Sequestrene Na₂, Édathamil, Éthylènediaminetétraacétate disodique dihydrate

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Changer de vue

A propos de cet article

Formule empirique (notation de Hill) :

C₁₀H₁₄N₂Na₂O₈ · 2H₂O

Numéro CAS:

6381-92-6

Poids moléculaire :

372.24

NACRES:

NA.77

PubChem Substance ID:

24894586

UNSPSC Code:

12352107

EC Number:

205-358-3

Beilstein/REAXYS Number:

3900609

Service technique

Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.

Laissez-nous vous aider

Propriétés

Quality Level

200

grade

ACS reagent

agency

suitable for SM 4500 - NH3

assay

99.0-101.0%

form

solid

reaction suitability

reagent type: chelator

impurities

≤0.005% Insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)

pH

4.0-6.0 (25 °C, 5%)

mp

248 °C (dec.) (lit.)

solubility

H₂O: 100 g/L at 20 °C

cation traces

Fe: ≤0.01%, heavy metals: ≤0.005% (by ICP-OES)

application(s)

microbiology

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

Description

General description

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Application

Chélateur de cations divalents. Inhibe les enzymes dont l′activité exige des cations divalents, comme les métalloprotéinases.

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:

of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Features and Benefits

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Still not finding the right product?

Explore all of our products under Acide éthylènediaminetétraacétique disodium salt dihydrate

pictograms

GHS08,GHS07

signalword

Warning

hcodes

H332,H373

pcodes

P260 - P271 - P304 + P340 + P312 - P314 - P501

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

target_organs

Respiratory Tract

Classe de stockage

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

Antibiotic Analysis Tetracyclines Sulfonamides Quinolones

LC-MS/MS-based determination of 19 sulfonamides, 13 quinolones, and 3 tetracycline drug residues in pork meat samples using Supel™ Swift HLB for extraction.

Contenu apparenté

Environmental Methods Consumables Look Up Tool

This page is intended to make it easier to find the consumables you need based on the analytical method you’re using. Methods included on this page come from the EPA, Standard Methods and ASTM.

Human Cardiomyocyte Progenitor Cells in Co-culture with Rat Cardiomyocytes Form a Pro-arrhythmic Substrate: Evidence for Two Different Arrhythmogenic Mechanisms

Smit NW, et al.

Frontiers in Physiology, 8, 797-797 (2017)

Mitophagy is required for mitochondrial biogenesis and myogenic differentiation of C2C12 myoblasts

Sin J, et al.

Autophagy, 12(2), 369-380 (2016)

Conformational landscape of the p28-bound human proteasome regulatory particle

Lu Y, et al.

Molecular Cell, 67(2), 322-333 (2017)

Afficher tous les articles scientifiques apparentés

Numéro d'article de commerce international

Référence	GTIN
E4884-100G	04061835544486
E4884-500G	04061835544493