E. coli Extract Polar

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.

E. coli Extract Polar is suitable to prepare small unilamellar vesicles, to mimic the surface of the E. coli inner membrane.

E. coli extract polar has been for the preparation of liposomes.

E. coli Extract Polar has been used:

• in unilamellar liposomes preparation for kinetic studies
• in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies
• in the reconstitution of molybdate transport protein (MolBC) for continuous wave-electron paramagnetic resonance studies

E.coli Polar lipid extracts comprise phosphatidylethanolamine as major lipid component. The minor components include the phosphatidylglycerol (PG) and cardiolipin. The leaflets of cytoplasmic membrane and inner leaflet of outer membrane are the source of E. coli lipid extract. It is used for reconstituting membrane proteins and forms bilayer by vesicle fusion.

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100600C-1g)

5 mL Clear Glass Sealed Ampule (100600C-100mg)

5 mL Clear Glass Sealed Ampule (100600C-500mg)

Avanti Research is a trademark of Avanti Polar Lipids, LLC